Abstract: Commonly, periodontal disease is caused by bacteria in subgingival plaque, such as Aggregatibacter actinomycetemcomitans and Treponema denticola. Rambutan leaf (Nephelium lappaceum L.) contains phenolic compounds that can act as antibacterial; therefore, it can be an alternative for chemical treatment of this disease. This study aimed to determine the effect of rambutan leaf extract against A. actinomycetemcomitans and T. denticola biofilm adhesion. This was an experimental and laboratory study using the post-test only control group design. The biofilm adhesion resistance test was done by using the biofilm assay method with crystal violet staining in 24 hours of incubation time. The material used was rambutan leaf extract in concentrations of 100%, 50%, 25%, 12.5%, 6.25%, and 3.125%. The optical density of biofilm was measured by using a microplate reader with a wavelength of 490 nm. Data were analyzed by using one-way ANOVA and Post-Hoc LSD (p<0.05). The results showed that rambutan leaf extract was most effective in inhibiting biofilm adhesion at 100% of concentration with an average optical density of 0.028 against A. actinomycetemcomitans, and at 50% concentration with an average OD of 0.120 against T. denticola. In conclusion, rambutan leaf extract can inhibit biofilm adhesion of Aggregatibacteractinomycetem- comitans and Treponema denticola.
Oleh :
Abdul Gani Soulissa
